Gas Chromatography-Mass Spectrometry

Procedure Title: Whole Oil /Extract Gas Chromatography Mass Spectrometry ( WOGCMS) (Diamondoids analysis)
Instrumentation:
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Trace 1310 Gas chromatograph with ISQ LT single quadrupole mass spectrometer
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Agilent (Hewlett Packard) HP 6890 GC /5973 MSD
Theory of Operation:

• Quantitative and qualitative analyses are performed on crude oils or extracts by injecting microliter volumes into a split/splitless injector. A GC capillary column is used to provide hydrocarbon separation. A mass selective detector provides ionization, fragmentation, filtration, and detection of diamondoids. GC-MS can be performed on whole oils saturate and aromatic fractions.

Analytical Procedure:

  • In a 2ml vial, take a known volume of the oil or the extract.

  • Add cholane (internal standard).

  • Add orthoterphenyl (OTP) (internal standard).

  • Add cyclohexane.

  • Cap the vial and mix it thoroughly.

  • Select the method and inject the oil into the GCMS according to the instruction manual.

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